Lukhan Tui (family) and Pete Samu (knee injury) both remained in Australia, with Ned Hanigan replacing Tui at blindside flank on Saturday.
Skipper Michael Hooper returns from a hamstring injury to start at openside and David Pocock is back at eight for the Port Elizabeth clash.
There is no specialist flanker on the bench but lock Rob Simmons can provide cover should the Wallabies require it.
Adam Coleman is the final change to the starting XV, teaming up with Izack Rodda in the second-row.
Coleman came off the bench for the Wallabies on the Gold Coast a fortnight ago but will start in Port Elizabeth, pushing Rory Arnold back to the bench.
As expected, Michael Cheika has opted to stick with the playmaking duo of Kurtley Beale and Matt Toomua in a backline that is unchanged from that which went up against the Pumas.
This Rugby Championship clash will also mark Scott Sio’s 50th Test appearance.
Australia: 15 Dane Haylett-Petty, 14 Israel Folau, 13 Reece Hodge, 12 Matt Toomua, 11 Marika Koroibete, 10 Kurtley Beale, 9 Will Genia, 8 David Pocock, 7 Michael Hooper (c), 6 Ned Hanigan, 5 Izack Rodda, 4 Adam Coleman, 3 Taniela Tupou, 2 Tatafu Polota-Nau, 1 Scott Sio
Replacements: 16 Folau Faingaa, 17 Sekope Kepu, 18 Allan Alaalatoa, 19 Rory Arnold, 20 Rob Simmons, 21 Nick Phipps, 22 Bernard Foley, 23 Jack Maddocks
Date: Saturday, September 29
Venue: Nelson Mandela Bay Stadium, Port Elizabeth
Kick-off: 17:05 local (16:05 BST, 15:05 GMT)
Referee: Jérôme Garcès (France)
Assistant referees: Wayne Barnes (England), Matthew Carley (England)
Television match official: Graham Hughes (England)
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