Treviso coach Umberto Casellato will be able to rely on the return of his international players for Saturday's game against the Ospreys.
Jayden Hayward is at full-back while on the wings are Ludovico Nitoglia and Andrea Pratichetti. Among the backs, Tommaso Iannone and Sam Christie will start in midfield.
A completely new half-back combination sees Edoardo Gori at scrum-half and James Ambrosini as fly-half.
Alberto De Marchi, Simone Ferrari and Davide Giazzon will make up the front row. The locks will be Duncan Naude and Jeff Montauriol. Finally Robert Barbieri, Dean Budd and captain Alessandro Zanni will complete the XV chosen by Casellato.
Director of Rugby Marius Goosen said: "We can't judge the Ospreys by their place in the table, if we analyse their matches they are playing really well and it will be a tough game as every year. But we know we are on the right track, just missing the result, for us and for our fans."
Meanwhile Ospreys head coach Steve Tandy said: "It's going to be another real test for us this weekend.
"Clearly we haven't had the start to the season that we would have wanted and when that happens, then it's easy to allow the pressure from outside to build.
"It's not going to get any easier, we have Treviso who can call on their Italian internationals again while next week we are home to Connacht who have only lost two players, but we know what we need to do."
Second row Lloyd Ashley will captain the side as Fiji's Josh Matavesi returns to start at inside centre.
Form: Something has to give as both of these sides have lost all three opening matches in the PRO12 this season. Benetton Treviso have not been victorious in the tournament since beating Cardiff Blues 40-24 at Stadio Monigo in February, which was also the last time they achieved a try bonus point. Ospreys have already become the first side in PRO12 history to begin campaign with three successive try-less games, and have in fact not scored a try for 267 minutes since Geoff Hassler crossed against Munster in last season's play-Off. Outside half Sam Davies has scored all Ospreys' 33 points, via 11 penalty goals, this season. The two sides met on four occasions last season with the Italian's solitary victory being 23-20 at Stadio Monigo in the Champions Cup in January.
Treviso: 15 Jayden Hayward, 14 Ludovico Nitoglia, 13 Tommaso Iannone, 12 Sam Christie, 11 Andrea Pratichetti, 10 James Ambrosini, 9 Edoardo Gori, 8 Robert Barbieri, 7 Dean Budd, 6 Alessandro Zanni (c), 5 Jeff Montauriol, 4 Duncan Naudè, 3 Simone Ferrari, 2 Davide Giazzon, 1 Alberto De Marchi
Replacements: 16 Luca Bigi, 17 Matteo Zanusso, 18 Salesi Manu, 19 Marco Fuser, 20 Francesco Minto, 21 Braam Steyn, 22 Chris Smylie, 23 Luke McLean
Ospreys: 15 Richard Fussell, 14 Tom Grabham, 13 Ben John, 12 Josh Matavesi, 11 Hanno Dirksen, 10 Sam Davies, 9 Brendon Leonard, 8 Dan Baker, 7 Sam Underhill, 6 Joe Bearman, 5 Rory Thornton, 4 Lloyd Ashley (c), 3 Dmitri Arhip, 2 Sam Parry, 1 Ryan Bevington
Replacements: 16 Scott Otten, 17 Gareth Thomas, 18 Cai Griffiths, 19 Rynier Bernardo, 20 Gareth Delve, 21 Tom Habberfield, 22 Jonathan Spratt, 23 Dan Evans
Date: Saturday, October 17
Venue: Stadio Monigo
Kick-off: 14:30 local (13:30 GMT)
Referee: Neil Hennessy (WRU)
Assistant Referees: Claudio Blessano, Giorgio Sgardiolo (both FIR)
TMO: Stefano Penne (FIR)