The Golden Lions will be captained by scrum-half Nic Groom for their final regular-season match of the Currie Cup season against the Pumas.
He starts alongside Springbok fly-half Elton Jantjies in the half-backs while Howard Mnisi and Lionel Mapoe form a strong midfield pairing.
The fifth-placed Pumas are four points behind the Golden Lions so will be desperate for a win that could see them sneak into the play-offs.
Meanwhile, a win for the visitors could lead to them finishing above the Blue Bulls, who host Western Province on Saturday, in third spot.
Golden Lions: 15 Sylvian Mahuza, 14 Wandisile Simelane, 13 Lionel Mapoe, 12 Howard Mnisi, 11 Courtnall Skosan, 10 Elton Jantjies, 9 Nic Groom (c), 8 Hacjivah Dayimani, 7 James Venter, 6 Hanko de Villiers, 5 Marvin Orie, 4 Rhyno Herbst, 3 Jacobie Adriaanse, 2 Corne Fourie, 1 Sti Sithole
Replacements: 16 Pieter Jansen, 17 Danie Mienie, 18 Chergine Fillies, 19 Driaan Bester, 20 Len Massyn, 21 Dillon Smit, 22 Shaun Reynolds
Date: Friday, October 12
Venue: Mbombela Stadium, Nelspruit
Kick-off: 19:00 local (18:00 BST, 17:00 GMT)
Referee: Rasta Rasivhenge
Assistant referees: Stephan Geldenhuys, Johre Botha
Television match official: Marius Jonker