England and Bath wing Anthony Watson will miss Saturday’s Six Nations encounter with rivals Scotland at Murrayfield because of a calf injury.
Watson sat out the 24-17 defeat to France in Paris with the same issue but it was expected that he would return for the trip to Edinburgh.
The back three player will now not be available for this weekend’s game, however, after suffering a recurrence of the problem in training.
Forwards coach Matt Proudfoot said: “Anthony felt it again yesterday and we won’t consider him for Scotland.”
Northampton Saints youngster George Furbank deputised in Watson’s absence but struggled as the Red Rose succumbed to Les Bleus.
Uncapped Gloucester wing Ollie Thorley could also step in after being considered for the match in Paris before Furbank was preferred.