Reds head coach Richard Graham has been forced to make a change to the team to face the Waratahs, with Mike Harris ruled out late due to an ankle injury.
Harris will be replaced by Anthony Fainga'a at inside centre, who will start his first game of the season for the Reds. Ben Lucas comes into the match-day 23 on the bench.
Harris pulled up fine from the Reds' 27-17 win over the Brumbies last weekend and trained well early in the week, but complained of ankle soreness after weights on Thursday.
He missed morning training to go for a scan on the ankle and has since been ruled out.
Reds (revised): 15 Aidan Toua, 14 Lachie Turner, 13 Ben Tapuai, 12 Anthony Fainga'a, 11 Dom Shipperley, 10 Quade Cooper, 9 Will Genia, 8 Jake Schatz, 7 Liam Gill, 6 Eddie Quirk, 5 James Horwill (c), 4 Rob Simmons, 3 Greg Holmes, 2 Saia Fainga'a, 1 James Slipper.
Replacements: 16 James Hanson, 17 Ben Daley, 18 Jono Owen, 19 Ed O'Donoghue, 20 Beau Robinson, 21 Nick Frisby, 22 Ben Lucas, 23 Chris Feauai-Sautia.
Date: Saturday, 1 March
Venue: ANZ Stadium in Sydney
Kick-off: 19:40 local time (08:40 GMT)
Referee: Glen Jackson (New Zealand)
Assistant referees: James Leckie (Australia), Will Houston (Australia)