Injuries have forced Chiefs coach Dave Rennie to name a new look backline for Friday night's Super Rugby opener against the Highlanders.
Andrew Horrell, Robbie Robinson, Brendon Leonard and Richard Kahui all remain out injured.
As a result, Tim Nanai-Williams will take the field at centre after a solid performance in last week's trial against the Hurricanes.
Chiefs midfielders Bundee Aki and Charlie Ngatai have both been cleared to play with the latter taking his place on the bench.
Aki as well as wing Patrick Osborne and hooker Rhys Marshall will all make their Super Rugby debuts.
Chiefs: 15 Gareth Anscombe, 14 Patrick Osborne, 13 Tim Nanai-Williams, 12 Bundee Aki, 11 Asaeli Tikoirotuma, 10 Aaron Cruden, 9 Tawera Kerr-Barlow, 8 Fritz Lee, 7 Sam Cane, 6 Liam Messam, 5 Brodie Retallick, 4 Craig Clarke, 3 Ben Afeaki, 2 Hika Elliot, 1 Pauliasi Manu.
Replacements: 16 Rhys Marshall, 17 Ben Tameifuna, 18 Michael Fitzgerald, 19 Tanerau Latimer, 20 Augustine Pulu, 21 Charlie Ngatai, 22 Lelia Masaga.
Date: Friday, February 22
Venue: Forsyth Barr Stadium, Dunedin
Kick-off: 19:35 (06:35 GMT)
Referee: Steve Walsh (Australia)
Assistant referees: Mike Fraser (New Zealand), Kane McBride (New Zealand)
Television match official: Vinny Munro (New Zealand)