Five uncapped players, two of them in the starting line-up, have been selected in the Springbok team to face Italy at Kings Park in Durban on Saturday.
Willie le Roux (full-back) and Jano Vermaak (scrum-half) will earn their Springbok debuts in the starting team, while the trio of uncapped players on the bench are Trevor Nyakane (prop), Arno Botha (loose forward) and Jan Serfontein (centre), who was part of the SA U20 team that won the Junior World Championship last year.
Stalwarts Bryan Habana (wing), Pierre Spies (number eight) and Tendai Mtawarira (prop), who missed the tour last November because of injury and health issues, are back in the starting team.
JJ Engelbrecht (centre) will start his first Test for South Africa at outside centre and Bjorn Basson (wing) will earn his seventh cap after last playing for the Springboks during the away leg of the Rugby Championships in 2011.
On the bench, Chiliboy Ralepelle (hooker), who also struggled with injuries last year, is back in the Test matchday squad. He will feature in his 22nd Test if he gets game time on Saturday.
Ten players who played in the Springboks' last Test of 2012, a 16-15 win over England at Twickenham, have been included in the match-23 for Saturday. They are Jean de Villiers, who is also the captain, Willem Alberts, Francois Louw, Juandré Kruger, Eben Etzebeth, Jannie du Plessis, Adriaan Strauss, Flip van der Merwe, Ruan Pienaar and Pat Lambie.
"This was not an easy team to select as I've been very impressed with the attitude shown by the players in training this week," said Springbok coach Heyneke Meyer on Wednesday.
"I'm very happy that we've managed to retain the core of the team from last year for the sake of continuity, but it's also great to see a bunch of new players emerge from Super Rugby.
"Up front we've managed to keep six of the eight who started against England and at the back there are a number of very exciting players who have earned their places in the team. I'm very excited to see what this new-look backline can do.
"A number of players just missed out on selection in the match-23, but there is no reason why they can't force their way into the reckoning next week."
Commenting on Saturday's Test, the first against Italy in almost three years, Meyer said: "We respect the Italians but the focus will be on what we want to achieve this weekend.
"In the past they have been regarded as one of the lesser Test nations, but that is no longer the case. Just look at their performances in the Six Nations this year - they beat Ireland and France in Rome and just lost to England in London.
"They have always been exceptionally strong upfront and will test us, especially at scrum time. But they also have some superb backs and are very well led by Sergio Parisse, so we know this will not be a walk in the park."
South Africa: 15 Willie le Roux, 14 Bryan Habana, 13 JJ Engelbrecht, 12 Jean de Villiers (c), 11 Bjorn Basson, 10 Morné Steyn, 9 Jano Vermaak, 8 Pierre Spies, 7 Willem Alberts, 6 Francois Louw, 5 Juandré Kruger, 4 Eben Etzebeth, 3 Jannie du Plessis, 2 Adriaan Strauss, 1 Tendai Mtawarira.
Replacements: 16 Chiliboy Ralepelle, 17 Trevor Nyakane, 18 Coenie Oosthuizen, 19 Flip van der Merwe, 20 Arno Botha, 21 Ruan Pienaar, 22 Pat Lambie, 23 Jan Serfontein.
Date: Saturday, June 8
Venue: Kings Park, Durban
Kick-off: 17.15 (15.15 GMT)
Referee: Pascal Gauzère (France)
Assistant referees: Nigel Hennessy (Wales), Blake Beattie (South Africa)