Former Springbok Hilton Lobberts will start alongside Ligtoring Landman for the Griquas in the opening game of Currie Cup 2013, against the Sharks.
Lobberts, centre Howard Mnisi, wing Nico Scheepers and full-back Gouws Prinsloo will all make their debut in the peacock-coloured jersey.
"All of the players who joined us from elsewhere have proven themselves over the last couple of weeks," coach Pote Human said in Kimberley.
"We have played a couple of warm up matches, two of which were against the Lions and Free State Cheetahs. They performed well there and we are looking forward to seeing the impact they make in the squad."
Lobberts joined the Northern Cape side after an unimpressive stint at Western Province, while Mnisi bagged a contract in Kimberley with his aggressive physicality for the Nelson Mandela Metropolitan University in this year's Varsity Cup competition.
Prinsloo joins the side after playing for the Sharks in the Vodacom Cup, while Scheepers switched from the Free State Cheetahs shortly after the Super Rugby competition.
Prinsloo would be under pressure to bolster Griquas in the wing position, left vacant by Willie le Roux who was called up to the Springbok squad for the Rugby Championship.
Prop Lourens Adriaanse, another Bok stalwart, was included in the Griquas side and would look to give the Springbok selectors something to mull over come scrum time.
The Kimberley-based side lost four of their regular players to other unions. Lock Edwinn Hewitt and flanker Justin Downey joined the Sharks while flyhalf Burton Francis moved to France to play club rugby for Agen.
Lock Martin Muller also opted to leave the central region for the Lions.
"We have managed to retain the core of the group which played in the Vodacom Cup," Human said.
"The absences of guys like Justin and Edwinn will be felt, and Willie le Roux's presence at the Springboks means we can't use him for Currie Cup rugby, but we have enough talented players to fill that void.
"The big challenge now is for us to go into the competition and show our intent early on. That will be the goal against the Sharks."
Among the regulars on the replacement bench, the coach included two other débutantes in Carel Greeff and Du Randt Gerber. Greeff would provide depth for the loose forwards while Gerber would cover fly-half Francois Brummer.
Griquas: 15 Gouws Prinsloo, 14 Nico Scheepers, 13 JP Nel, 12 Howard Mnisi, 11 PJ Vermeulen, 10 Francois Brummer, 9 Jacques Coetzee, 8 Burger Schoeman, 7 Jaco Negpen, 6 Marnus Schoeman, 5 Ligtoring Landman, 4 Hilton Lobberts, 3 Lourens Adriaanse, 2 Ryno Barnes (capt), 1 Steph Roberts.
Replacements: 16 Matthew Dobson, 17 Nick Schonert, 18 Jonathan Adendorff, 19 Carel Greeff, 20 Marnus Hugo, 21 Du Randt Gerber, 22 Rocco Jansen.
Date: Friday, August 9
Kick-off: 19:10 local (18:10 BST, 17:10 GMT)
Venue: Kings Park, Durban
Referee: Jason Jaftha
Assistant referees: Rasta Rasivhenge, Reuben Rossouw
Television match official: Willie Roos