Champions Harlequins open the defence of their LV= Cup title in Round One away to Exeter Chiefs over the weekend of 8, 9 and 10 November.
Quins have been drawn in Pool Three with Bath, Worcester Warriors and Ospreys for the 2013/14 LV= Cup.
In the format of the LV= Cup, teams drawn in Pool One play teams in Pool Four, while teams drawn in Pool Two play teams in Pool Three.
This means Quins will compete against Exeter Chiefs, Cardiff Blues, Leicester Tigers and last year's finalists, Sale Sharks.
Round Two sees a repeat of the 2013 LV= Cup final as Harlequins play Sale at the Stoop over the weekend of 15, 16 and 17 November.
Harlequins secured the title back in March 2013, lifting the LV= Cup at Sixways Stadium following a comprehensive 32-14 victory over the Sharks.
Last season's semi-finalists Saracens will begin their new season campaign against London Wasps. The men of Adams Park will be looking to improve on their 2012/13 standing, having come last in Pool 3 with one victory over Worcester Warriors.
Elsewhere in the competition, Scarlets will go head-to-head with fellow Welsh region the Dragons, with both teams finishing third in their respective pools last season.
Headed up by former London Welsh Head Coach Lyn Jones, the Dragons will travel to Parc y Scarlets, home of winning British and Irish Lions centre Jonathan Davies, in Round One of the competition.
Credited for playing a pivotal role in the development of players in the elite game, the LV= Cup has sought to recognise outstanding talent through the LV= Cup Breakthrough Player Award.
Inaugural winner Jonny May (Gloucester) has since been selected in England's summer tour to Argentina, whilst last season's winner Jack Nowell (Exeter Chiefs) lifted the Junior World Championship trophy with England U20s last month.
Following the fixture announcement, RFU Professional Rugby Director Rob Andrew commented: "We have seen the LV= Cup develop as a crucial competition for up and coming new talent over the past few seasons as evident through players such as Jack Nowell, who was crucial in England U20's Junior World Championship triumph.
"I'm sure the competition will again identify some promising young talent this season, and ones that we will undoubtedly see on the international stage in the years to come."
The top team from each pool will qualify for the semi-finals which are held on the weekend of 7, 8 and 9 March.
The final will be played on Sunday 16 March. The venue for which will be confirmed in coming months.
Pool One: Saracens, Gloucester, London Irish, Dragons.
Pool Two: Leicester Tigers, Exeter Chiefs, Sale Sharks, Cardiff Blues.
Pool Three: Harlequins, Bath, Worcester Warriors, Ospreys.
Pool Four: Northampton Saints, London Wasps, Newcastle Falcons, Scarlets.
Round One - 8, 9 and 10 November: London Wasps v Saracens, Northampton Saints v Gloucester, Newcastle Falcons v London Irish, Scarlets v Dragons, Exeter Chiefs v Harlequins, Leicester Tigers v Ospreys, Sale Sharks v Bath, Cardiff Blues v Worcester Warriors.
Round Two - 15, 16 and 17 November: London Irish v Northampton Saints,Dragons v London Wasps, Saracens v Scarlets, Gloucester v Newcastle Falcons, Bath v Exeter Chiefs, Worcester Warriors v Leicester Tigers, Harlequins v Sale Sharks, Ospreys v Cardiff.
Round Three - 24, 25 and 26 January: Scarlets v Gloucester, London Wasps v London Irish, Saracens v Newcastle Falcons, Dragons v Northampton Saints, Bath v Cardiff Blues, Exeter Chiefs v Ospreys, Harlequins v Leicester Tigers, Sale Sharks v Worcester Warriors.
Round Four - 31 January, 1 and 2 February: Gloucester v London Wasps, London Irish v Scarlets, Newcastle Falcons v Dragons, Northampton Saints v Saracens, Leicester Tigers v Bath, Worcester Warriors v Exeter Chiefs, Cardiff Blues v Harlequins, Ospreys v Sale Sharks.
Semi Finals - 7, 8 and 9 March:
The pool winner with the best playing record will be at home against the pool winner with the fourth best playing record in Semi Final 1.
The pool winner with the second best playing record will be at home against the pool winner with the third best playing record in semi Final 2.
LV= Cup Final - 16 March:
Final venue to be confirmed.