Wallabies hooker Stephen Moore has been ruled out of Saturday's fixture against the Springboks in Perth after injuring his hamstring at training.
Moore was on Thursday sent for scans to determine how serious the damage was. He is now sidelined and will be replaced by Tatafu Polota-Nau while Saia Faingaa slots in as the back-up hooker on the replacements bench.
Australia prop Ben Alexander said preparations have not been affected by the withdrawal.
"We've been practicing a lot at training with lots of different combinations, we very rarely pack more than a couple in a row with the same guys," he told AAP.
"We're always changing props, changing sides, changing hookers, so it shouldn't cause any problem."
What's even more disappointing for Moore is that had he played, the Brumby would have overtaken Jeremy Paul as the most capped Wallabies hooker of all time.