La Unión Argentina de Rugby (UAR) has confirmed the Pumas' home clash with South Africa on Saturday, August 15 will be played at Vélez Sarsfield.
The game in Buenos Aires will kick-off at 16:40 local time.
South Africa have visited the stadium on two occasions, posting a 39-7 win in December 2004 and 34-23 in November 2005 while Los Pumas have recorded eleven wins, fifteen defeats and one draw there since the venue's first Test in 1986.
The stadium is situated in the west of the Argentine capital and has previously hosted 27 Tests, the most recent of which was England's victory in June 2013.
Not only does the international match serve as the last opportunity for fans to bid farewell to Los Pumas ahead of the Rugby World Cup, but it also marks the 50th anniversary of Argentina's 1965 tour of South Africa and the UAR will celebrate the occasion with all living players and personnel.