Cape Town hosts South Africa and defending Series champions Fiji are both through to Sunday's Cup quarter-finals.
The Blitzboks will take on Wales having won all three of their matches on Day 1 against Russia, the USA and Australia.
Fiji topped Pool B after also finishing unbeaten on Saturday, while New Zealand pipped England to top spot in Pool C after two wins and a draw in there three matches.
Scotland were the final pool winners thanks to their three wins in Pool D up against Wales, Samoa and Uganda.
Kick-off times are GMT +2
South Africa v Wales – 12:04
New Zealand v Kenya – 12:26
Scotland v USA – 12:48
Fiji v England – 13:10
After last week’s Dubai Sevens, the Blitzboks will be looking to defend their Cape Town Sevens title on day two and increase their lead at the top of the series standings after the opening two rounds.
After the last match of the day, South African captain Philip Snyman said: "It's been a good week for us. There are a lot of things here in Cape Town that can distract us but, over the last few days, we put last week's win in Dubai behind us. The guys played some nice rugby here today and I think we'll keep on building."
On a day when England speedster Dan Norton became England's top scorer, and third-highest try scorer in sevens history behind Argentina's Santiago Gomez Cora and Kenya's Collins Injera, Canada's captain John Moonlight appeared in his 50th tournament as did Kenya's skipper, Andrew Amonde.
Last week’s Dubai champions, hosts and defending champions in Cape Town South Africa got off to a flying start as World Rugby sevens player of 2016 Seabelo Senatla and Werner Kok both scored twice for the Blitzboks in their 41-0 win over Russia.
In their second match against last week’s Challenge Trophy winners USA, Maka Unufe gave the Americans the lead but tries from Cecil Afrika, Kok (2) and Senatla secured the 28-10 win for Neil Powell’s side.
The South Africans rounded off a successful day on home soil with a 29-7 win against Australia, with Cecil Afrika and Nel each getting a try and captain Philip Snyman scoring two.
USA, captained by Perry Baker for the first time, started with a 19-0 win against Australia with Baker opening the scoring, before beating Russia 26-17 thanks to tries from Danny Barrett, Stephen Tomasin and Baker (2) to reach the last eight.
Andy Friend’s Australia followed their defeat to the USA, beating Russia 36-0, but this was not enough to put them into the last eight and they will now compete in the Challenge Trophy.
Fiji defeated Japan with five different try scorers crossing the whitewash in their 33-7 victory, before beating Kenya 28-22 with conversions proving decisive as both sides scored four tries.
The last time the sides met was the HSBC Singapore Sevens final where Kenya won their first-ever sevens tournament, but they could not repeat the result as they lost to the Olympic gold medallists 28-22. after two tries from the impressive Samisoni Viriviri.
In Kenyan captain Andrew Amonde’s 50th tournament, he inspired his side to an opening 33-14 win over France with Billy Odhiambo and Willy Ambaka both scoring two tries each before their defeat to Fiji. They confirmed their spot in the last eight as they defeated Japan 24-5 with tries from Augustine Lugonzo, Odhiambo (2) and Ambaka.
Fiji captain Osea Kolinisau opened the scoring in Fiji’s third pool match, to help them top Pool B, before Viriviri, Setareki Bituniyata and Jerry Tuwai confirmed a narrow 28-19 win over France.
France bounced back from their opening defeat to Kenya with an impressive record 50-7 win over Japan. Terry Bouhraoua and Manoel Dall’Igna both scored twice but it was not enough as they suffered defeat to Fiji.
England’s Dan Norton and James Rodwell both broke records on day one as their side reached the quarter-finals. First Rodwell became the most-capped England sevens player with a 71st tournament appearance before Norton scored his 220th, 221st and 22nd tries to become the all-time leading try scorer for England.
Taking over from Ben Gollings (220), Norton also moved into third on the all-time list behind Collins Injera (235) and Santiago Gomez Cora (230). England opened with a 33-10 win over Canada before Norton scored a brace in their 19-14 win over Argentina.
Having finished eighth last week in Dubai, New Zealand looked an improved side early on, beating Argentina 26-12 before Canada came from 14-0 behind to draw 19-19 against the All Blacks Sevens. John Moonlight scored one of the Canadian’s three tries in his 50th appearance against New Zealand.
Simon Amor’s England missed out on top spot in the pool with a defeat at the hands of the All Blacks Sevens. Captain Tom Mitchell had got the scoring underway, but Sherwin Stowers’ 113th try, Tim Mikkelson’s third try of the day, two DJ Forbes efforts and a penalty try secured an impressive 33-7 win and top spot for the Kiwis.
New Zealand's Tim Mikkelson was happy with his side's performance, especially after beating England in the last Pool C match: "We played (England) last weekend in the last game and they gave us a bit of a lesson. We knew going into that game that we had probably had to win to make it to the cup so we're really pleased with that performance."
Bautista Delguy impressed in Argentina’s third pool match, scoring a first-half hat-trick against Canada as Santiago Gomez Cora’s side won 43-14 to finish third in Pool C.
Scotland came from 19-7 behind in the opening match of the Cape Town Sevens to beat Samoa, with James Fleming drawing the scores level after the buzzer had sounded.
Scott Wight stepped up to kick a difficult conversion to give Scotland the 21-19 win, before they beat Uganda 38-7 in their second match to book a place in the Cup quarter-finals thanks to two tries from Jamie Farndale.
Wales joined them in the quarter-finals for a second successive week after they beat Uganda 29-7 in their first match before a tight encounter with Samoa. Matthew Owen scored a try in each half before Sam Cross secured the win for last week’s fourth placed side.
Samoa finished third in Pool D with a win over Uganda with Alatasi Tupou scoring twice in the 29-5 win.
In the pool decider Scotland beat Wales 24-21 in a tight encounter. Wales were down to five men in the first half as Scotland opened up a 19-0 lead. Two second half tries from Owen Jenkins had given the Welsh a glimpse but the Scots held out to top the pool.
Challenge Trophy quarter-finals
Australia v Uganda – 10:36
Argentina v Japan – 10:58
Samoa v Russia – 11:20
France v Canada – 11:42
Day One Results
Scotland 21-19 Samoa
Wales 29-7 Uganda
New Zealand 26-12 Argentina
England 33-10 Canada
France 14-33 Kenya
Fiji 33-7 Japan
Australia 0-19 USA
South Africa 41-0 Russia
Scotland 38-7 Uganda
Wales 17-0 Samoa
New Zealand 19-19 Canada
England 19-14 Argentina
France 50-7 Japan
Fiji 28-22 Kenya
Australia 36-0 Russia
South Africa 28-10 USA
Samoa 29-5 Uganda
Wales 21-24 Scotland
Argentina 43-14 Canada
England 7-33 New Zealand
Kenya 24-5 Japan
Fiji 28-19 France
USA 26-17 Russia
South Africa 29-7 Australia