Argentina fly-half Nicolás Sánchez and wing Manuel Montero have been ruled out of Saturday's game against Australia.
The duo were initially named in Daniel Hourcade's side on Thursday but have both been withdrawn following Argentina's captain's run at Twickenham.
As a result Santiago González Iglesias moves to fly-half, with Jerónimo de la Fuente coming in at inside centre to form a partnership with Matiás Orlando.
Montero meanwhile is replaced on the wing by Ramiro Moyano. Gabriel Ascarate and Lucas González Amorosino are both confirmed on the bench in the 22 and 23 jerseys.
Argentina: 15 Joaquín Tuculet, 14 Matías Moroni, 13 Matías Orlando, 12 Jerónimo De la Fuente, 11 Ramiro Moyano, 10 Santiago González Iglesias, 9 Martín Landajo, 8 Leonardo Senatore, 7 Javier Ortega Desio, 6 Pablo Matera, 5 Matías Alemanno, 4 Guido Petti, 3 Ramiro Herrera, 2 Agustín Creevy, 1 Lucas Noguera
Replacements: 16 Julian Montoya, 17 Santiago García Botta, 18 Enrique Pieretto, 19 Marcos Kremer, 20 Juan Manuel Leguizamon, 21 Tomás Cubelli, 22 Gabriel Ascarate, 23 Lucas González Amorosino
Date: Saturday, October 8
Venue: Twickenam Stadium, London
Kick-off: 19:30 local (18:30 GMT)
Referee: Mathieu Raynal (France)
Assistant Referees: JP Doyle (England), Matthew Carley (England)
TMO: Rowan Kitt (England)