The Springboks arrived in Brisbane on Monday after travelling in two groups to Queensland, where they will take on the Wallabies this weekend.
The squad of 31 players and five management flew from Johannesburg via Sydney while the rest of the management travelled via Perth to Brisbane.
South Africa's medical team will make sure every member of the travelling squad is fit and raring to go before the first training run on Tuesday.
"We've usually travelled to Australia on a bye weekend, giving us one extra day to prepare, but we don't have it this year and we'll have to make sure we hit the ground running on Tuesday," said Springbok coach Heyneke Meyer.
"We worked on a couple of new things since we got together and the match against the World XV was a good barometer to see where we stand. The challenge this week would be to improve on certain aspects of the game and to fine tune others.
"We need to make a big step-up against the Wallabies. It will be a very high tempo game and proper Test match rugby against one of the top sides of the world.
"They had two teams in the semi-finals of Super Rugby this year, which probably points to more depth in their structures, but I saw since the day we got together a passion and excitement amongst our players to make our country proud."
Meyer will name his matchday 23 to play at Suncorp Stadium on Wednesday. It's a venue that has not been kind to the Springboks in recent years, although South Africa scored a brilliant 38-12 victory over the Wallabies in Brisbane in 2013.
"Before that match, the Springboks had never beaten Australia at Suncorp Stadium and although the match was played two years ago, we'd like to build on that performance," said Meyer.
"They are always a tough opponent at home – since the start of the Tri-Nations in 1996 and later in the Rugby Championship, the Springboks have won only three out of 23 matches against the Wallabies in Australia, drawing one and losing 18.
"This was also clear last year when we played very well, but they managed to beat us with the last movement of the match in Perth."