Saracens and England second row George Kruis has been ruled out of the 2017 Six Nations through injury.
Kruis will undergo surgery on Tuesday, February 7 for a knee ligament injury sustained in training last week.
It is expected that the 26-year-old, who has 20 international caps, will require a 10-12 week rehabilitation period.
Meanwhile, 32 players have assembled at Pennyhill Park on Sunday to train for Saturday’s Six Nations game against Wales at the Principality Stadium.
Prop Mako Vunipola (Saracens) will also join-up with England this week and will continue his rehabilitation from injury.
England’s Six Nations squad
Forwards (18): Jack Clifford (Harlequins), Dan Cole (Leicester Tigers), Charlie Ewels (Bath), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Teimana Harrison (Northampton Saints), Dylan Hartley (Northampton Saints), James Haskell (Wasps), Nathan Hughes (Wasps), Maro Itoje (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Joe Marler (Harlequins), Matt Mullan (Wasps), Kyle Sinckler (Harlequins), Tommy Taylor (Wasps), Mike Williams (Leicester Tigers), Tom Wood (Northampton Saints)
Backs (14): Mike Brown (Harlequins), Danny Care (Harlequins), Elliot Daly (Wasps), Owen Farrell (Saracens), George Ford (Bath), Jonathan Joseph (Bath), Alex Lozowski (Saracens), Jonny May (Gloucester), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Ben Te’o (Worcester Warriors), Anthony Watson (Bath Rugby), Marland Yarde (Harlequins), Ben Youngs (Leicester Tigers)