PRO12 champions Connacht will host Glasgow Warriors in a repeat of last season's semi-final when the new season starts in September.
Last season's runners-up Leinster start the new season on Friday, September 2 at home to Treviso.
Two other fixtures on that Friday will see the Ospreys begin their campaign at the Liberty Stadium up against Zebre, while Ulster face Newport Gwent Dragons at the Kingspan Stadium.
Aside from Connacht's clash with Glasgow on the Saturday, Scarlets take on Munster while Cardiff Blues complete the fixtures at home to Edinburgh.
PRO12 Round One Fixtures (all kick-off times are local)
Friday, September 2
Leinster v Treviso (19:35)
Ospreys v Zebre (19:35)
Ulster v Dragons (19:35)
Saturday, September 3
Scarlets v Munster (15:00)
Connacht v Glasgow Warriors (17:15)
Cardiff Blues v Edinburgh (19:35)