Scrum-half Tian Meyer will captain the Cheetahs against the Jaguares at Vélez Sarsfield in Buenos Aires on Saturday.
Coach Franco Smith has made several changes to his side that beat the Sunwolves last week.
The front row has been overhauled with Danie Mienie, Elandré Huggett and Tom Botha taking the field.
In the second row Armandt Koster replaces Justin Basson in the four jersey while Henco Venter rotates with Paul Schoeman dropping to the bench. Ryno Benjamin moves from outside centre to 14.
There are a handful of players new to the bench this week with Joseph Dweba, Carl Wegner, William Small-Smith, Boom Prinsloo and Shaun Venter all providing cover.
Cheetahs: 15 Clayton Blommetjies, 14 Ryno Benjamin, 13 Michael van der Spuy, 12 Clinton Swart, 11 Ruan van Rensburg, 10 Niel Marais, 9 Tian Meyer (c), 8 Niell Jordaan, 7 Oupa Mohoje, 6 Henco Venter, 5 Francois Uys, 4 Armandt Koster, 3 Tom Botha, 2 Elandré Huggett, 1 Danie Mienie
Replacements: 16 Joseph Dweba, 17 Charles Marais, 18 Johan Coetzee, 19 Carl Wegner, 20 Paul Schoeman, 21 William Small-Smith / Boom Prinsloo, 22 Shaun Venter, 23 Fred Zeilinga
Date: Saturday, March 18
Venue: Vélez Sarsfield, Buenos Aires
Kick-off: 16:40 local (19:40 GMT)
Referee: Paul Williams (New Zealand)
Assistant Referees: Nick Briant (New Zealand), Pablo Deluca (Argentina)
TMO: Santiago Borsani (Argentina)