Further medical assessment to George Kruis (Saracens) confirmed that the second-row has sustained a knee ligament injury.
Kruis will see a specialist on Thursday to determine further management of the injury.
England head coach Eddie Jones commented, “We’ll have to wait and see what the specialist’s prognosis is, but we’re not ruling him out of the Six Nations at this stage.”
Kruis has been expected to start alongside Courtney Lawes in England’s second row against France, with Maro Itoje moving to blindside flanker.
England will name their team for the Six Nations opener on Thursday.